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ReadyToProcess WAVE 25 Rocker

The ReadyToProcess WAVE 25 rocker features integrated temperature sensors and load cells, designed for convenient handling and control of cell cultures up to 25 L. A filter heater is delivered with the system.
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Detailed introduction

The ReadyToProcess WAVE 25 rocker features integrated temperature sensors and load cells, designed for convenient handling and control of cell cultures up to 25 L. A filter heater is delivered with the system.

       Ergonomic design makes handling convenient and easy

       Minimal footprint facilitates placement when space is limited

       Ability to handle the bioreactor in a tilt position facilitates sampling and harvest

       Operation in single or dual culture mode

       Reliable temperature measurement from integrated sensors

       Accurate weight measurement from integrated load cells

 

Mixing and gas transfer

The adjustable rocking parameters are speed, angle, and motion. The parameter settings, in combination with the cell culture volume, affect the mixing and gas transfer rate in the Cellbag bioreactor. The speed parameter determines the number of rocking cycles per minute, and the angle parameter relates to the tray’s degree of tilting at the turning points. The motion parameter determines the acceleration profile.

 

Temperature control

Reliable, efficient, and evenly distributed heating is provided by the tray heater plate. The temperature measurement is managed by sensors integrated in the rocker base unit. For an accurate, stable, and fast temperature control, the heater power output is automatically adjusted based on the Cellbag bioreactor size and the medium volume as measured by the internal scale. When used in dual mode, temperature is controlled independently in the two separate Cellbag bioreactors.

 

Weight measurement

Load cells integrated in the rocker provide accurate and continuous weight measurement of the culture and eliminate the need for external scales. The load cells communicate with the system control function for proper medium handling and for tailored regulation of pH and temperature. The weight measurement functionality is also used for automatic calibration of the pump when used in perfusion culturing. Adjustable feet enable equal weight distribution between the load cells also on non-level surfaces, for increased precision of the weight measurement.

 

Data storage and communication

All run data and control parameters are stored on two independent, mirrored solid state drives integrated in the rocker. After a run, data is stored in a UNICORN database on an external computer. For facilitated integration into a larger manufacturing operation, the rocker also contains software to enable direct communication with the system via an open platform communication (OPC) link.

 

Trays and lids

Trays are available in three different sizes for culturing of up to 5 L, 10 L, and 25 L. The trays are easily attached to the rocker when in the tilt position. A snap lock mechanism allows Cellbag bioreactors to be correctly installed and rapidly changed. To protect light-sensitive components of the culture medium and to prolong the life of the optical sensors, lids are available for all tray sizes. The ergonomic rocker design makes handling convenient and easy. The ability to handle the bioreactor in a tilt position facilitates sampling and harvest. The wide top opening facilitates for the user to interact with the culture without removing the lid.